things to ask SUe or Kathy਀
4.1਀琀漀 焀甀愀渀琀椀昀礀 琀栀攀 刀一䄀 愀猀欀 䬀 昀漀爀 伀䐀 瘀愀氀甀攀
਀愀猀欀 匀唀攀 昀漀爀 猀瀀攀挀椀昀椀挀猀 愀戀漀甀琀 琀栀攀 儀椀愀最攀渀 欀椀琀
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what is the composition of the RT-PCR buffer that we used instead of 5μL of 2X DNase buffer, is it the same as 5X RT buffer?਀
can I mention the addition of the master mix components seperately?਀
Which stuff should I put final concentrations for?਀
where did the cDNA, genomic and RNA come from for PCR? stupid question! they all came from E.coli!਀
4.3਀搀椀氀甀琀攀搀 吀㐀 瀀漀氀礀渀甀挀氀攀漀琀椀搀攀 欀椀渀愀猀攀℀℀㼀 眀栀愀琀猀 琀栀攀 挀漀渀挀攀渀琀爀愀琀椀漀渀㼀㼀㼀℀℀℀℀
਀猀栀漀甀氀搀 䤀 洀攀渀琀椀漀渀 琀栀攀 洀愀猀琀攀爀 洀椀砀 漀爀 椀琀猀 挀漀洀瀀漀渀攀渀琀猀
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do we actually go through the steps of preparing a sequencing gel even tho we didn't do it? or do we just say samples run on gel at 60 watts for 2 hrs?