ProtParam tool
User-provided sequence:
1 11 21 31 41 51
| | | | | |
1 ATGGCCGAAT CGGACAAAAT AAACATCGAT TCGATCATCC AGCGCMAESD KINIDSIIQR 60
61 CTTTTGGAAG TGAAGGGCTC AAGACCTGGT AAAAATGTTC AGTTALLEVK GSRPGKNVQL 120
121 ACTGAGCAAG AGATTCGAGG ATTGTGTCTA AAATCTCGAG AAATCTEQEI RGLCLKSREI 180
181 TTTCTTAGTC AGCCAATACT ACTGGAGCTG GAGGCACCCC TCAAGFLSQP ILLELEAPLK 240
241 ATTTGTGGTG ATGTTCATGG TCAGTACTAT GACCTGCTCA GGCTTICGDV HGQYYDLLRL 300
301 TTTGAGTATG GAGGATATCC ACCAGAGAGT AACTACCTAT TTCTGFEYGG YPPESNYLFL 360
361 GGAGACTATG TTGACAGAGG CAAGCAGTCA CTGGAGACCA TCTGCGDYVD RGKQSLETIC 420
421 CTGCTGCTTG CTTACAAGAT CAAATATCCA GAGAACTTCT TTCTTLLLAY KIKYPENFFL 480
481 CTCAGAGGAA ACCATGAATG TGCCTCAATC AACAGGATAT ATGGALRGNH ECASINRIYG 540
541 TTTTATGATG AATGTAAAAG ACGTTATAAC ATCAAGCTCT GGAAAFYDEC KRRYNIKLWK 600
601 ACATTCACAG ACTGTTTCAA CTGCTTGCCA GTAGCTGCTA TTGTGTFTDC FNCLPVAAIV 660
661 GATGAAAAGA TTTTTTGTTG TCATGGAGGT CTTTCTCCAG ACCTGDEKIF CCHGGLSPDL 720
721 CAATCAATGG AACAGATTCG GCGTGTGATG AGGCCTACGG ATGTCQSMEQ IRRVMRPTDV 780
781 CCTGACCAGG GTTTGCTGTG TGATCTGCTC TGGGCTGACC CTGATPDQGL LCDLLWADPD 840
841 AAAGATGTCA TGGGCTGGGG AGAGAATGAT CGTGGTGTTT CCTTCKDVMG WGENDRGVSF 900
901 ACCTTCGGGT CTGATGTCGT GGCCAAGTTT TTGCACAAAC ATGACTFGSD VVAKFLHKHD 960
961 ATGGATCTCA TATGCAGAGC CCATCAGGTG GTGGAGGATG GCTACMDLIC RAHQVVEDGY 1020
1021 GAGTTCTTTG CGAAGAGGCA GTTAGTCACG CTTTTCTCTG CTCCCEFFAK RQLVTLFSAP 1080
1081 AACTACTGCG GCGAGTTTGA CAATGCAGGT GCCATGATGA GTGTTNYCGE FDNAGAMMSV 1140
1141 GATGAGACCC TTATGTGCTC ATTTCAGATT CTGAAACCAG CAGAGDETLM CSFQILKPAE 1200
1201 AAGAAGGTGC TGTCATATGG CGGCGCTGGA GGATTTGGGA ACAGCKKVLS YGGAGGFGNS 1260
1261 AGACCTGTAA CTCCTCCTCG GAACACAGCC AAGGGTGGCA AAGCCRPVTP PRNTAKGGKA 1320
1321 AAGAAATAAK K
References and documentation are available.
Number of amino acids: 1331
Molecular weight: 119662.5
Theoretical pI: 6.09
Amino acid composition:
Ala (A) 285 21.4%
Arg (R) 18 1.4%
Asn (N) 13 1.0%
Asp (D) 23 1.7%
Cys (C) 219 16.5%
Gln (Q) 12 0.9%
Glu (E) 21 1.6%
Gly (G) 285 21.4%
His (H) 6 0.5%
Ile (I) 18 1.4%
Leu (L) 36 2.7%
Lys (K) 23 1.7%
Met (M) 8 0.6%
Phe (F) 18 1.4%
Pro (P) 16 1.2%
Ser (S) 17 1.3%
Thr (T) 278 20.9%
Trp (W) 3 0.2%
Tyr (Y) 14 1.1%
Val (V) 18 1.4%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 41
Atomic composition:
Carbon C 4691
Hydrogen H 7636
Nitrogen N 1448
Oxygen O 1754
Sulfur S 227
Formula: C4691H7636N1448O1754S227
Total number of atoms: 15756
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 52305 50243 48890 48070 46680
Abs 0.1% (=1 g/l) 0.437 0.420 0.409 0.402 0.390
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 36500 36400 35810 34990 33600
Abs 0.1% (=1 g/l) 0.305 0.304 0.299 0.292 0.281
Estimated half-life:
The N-terminal of the sequence considered is A (Ala).
The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.17
This classifies the protein as unstable.
Aliphatic index: 41.16
Grand average of hydropathicity (GRAVY): 0.465
ExPASy Home page